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For where To Order Aristocort Online Safe reasons we prefer polyethylene tubes with screw caps, even if the iodination is equally efficient when carried out in glass tubes. Since maximum incorporation is obtained after 7—13 min, we have routinely used a reaction time of 10 min. The apparent decrease in incorporation after 15—30 min could possibly be due to the formation of aggregates unable to enter the gel filtration column.
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The parallelism of the self-displacement curves and standard curves in addition to the high percentage of labelled antigen bound in the presence of antibody excess suggest retained immunological activity. Their lack of success with Iodogen might be explained by the enormous molar excess of Iodogen and radioactive iodide used. Our experience strongly supports the view that Iodogen provides an effective, gentle, and simple way to iodinate proteins and polypeptides. One speaker reported poor results with iodination times of 20-60 s at room temperature. Another speaker reported more successful results with iodination times of 20-30 min at room temperature.
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